The following pages will contain photographs of various ANA patterns. These photomicrographs were taken of actual patient samples using indirect immunofluorescence (IFA) and the Colorzyme® methods.
As described previously, the Colorzyme® method uses a horseradish peroxidase (HRP) conjugate, and the results are read using brightfield microscopy.
In an effort to standardize the interpretation of ANA patterns, the Autoantibody Standardization Committee*, a subcommittee of the International Union of Immunological Societies (IUIS) Quality Assessment and Standardization Committee in conjunction with the Centers for Disease control and Prevention (CDC), formed the International Consensus on ANA Patterns** (ICAP). This group has issued a standardized set of ANA photographs for nuclear, cytoplasmic, and mitotic HEp-2 cell patterns designated as AC-1 through AC-28.
Reference: Chan EK, Damoiseaux J, Carballo OG, Conrad K, de Melo Cruvinel W, Francescantonio PL, Fritzler MJ, Garcia-De La Torre I, Herold M, Mimori T, Satoh M, von Mühlen CA, Andrade LE. Report of the first international consensus on standardized nomenclature of antinuclear antibody HEp-2 cell patterns 2014-2015.
Front Immunol. Aug. 20, 2015; 6:412. Available at:
http://journal.frontiersin.org/article/10.3389/fimmu.2015.00412/full. Accessed March 29, 2018.
** The International Consensus on ANA Patterns (ICAP) is available at:
www.anapatterns.org. Accessed March 29, 2018.
