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The page below is a sample from the LabCE course Proficiency Test Handling and Communication. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Testing Preparation

CLIA regulations require that PT samples be tested in an identical manner to patient samples. Sample preparation for PT samples may be required and there may be slight alterations in the evaluation process due to suspension medium or origin of sample contents. Failure to comply with kit instructions may lead to aberrant or incorrect test results that may be unacceptable and result in event failure.
Prior to any testing, perform the following steps:
  • Read the read kit instructions before beginning.
  • Reconstitute samples as specified in the kit instructions.
  • Follow all handing instructions, including warming, mixing, and test mode for performance.
  • Follow patient testing protocol, as per policy and procedure, to include the number of test repetitions, critical value policy, and reporting units.