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The page below is a sample from the LabCE course Overview Of Major Antigens of the Rh Blood Group System. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Rh Case Study, Further Testing

Based on the positive antibody screen results, the patient's sample was tested against a commercially prepared panel of group O red cells. Indirect antiglobulin testing was performed in the tube using LISS enhancement media. The following antigram shows results obtained when this testing was performed. Using crossout or process of elimination techniques, interpret the most probably alloantibody demonstrated.
Rh Phenotype
D
C
c
E
e
K
k
Fya
Fyb
Jka
Jkb
Lea
Leb
P1
M
N
S
s
Patient Results
IS
LISS
AHG
√ cells
RzR1
1
+
+
0
+
+
0
+
+
0
+
+
0
+
+
+
0
0
+
0
2+
NT
R1R1
2
+
+
0
0
+
+
+
+
+
+
+
0
+
0
+
+
0
+
0
2+
NT
R2R2
3
+
0
+
+
0
0
+
0
+
0
+
0
+
+
0
+
0
+
0
0
R0
4
+
0
+
0
+
0
+
0
0
+
0
0
0
+
+
+
+
0
0
0
r’r
5
0
+
+
0
+
0
+
0
+
0
+
+
0
+
+
0
+
+
0
1+
NT
r”r
6
0
0
+
+
+
0
+
0
+
+
0
0
+
0
+
0
+
+
0
0
rr
7
0
0
+
0
+
+
+
0
+
0
+
0
+
+
0
+
+
+
0
0
rr
8
0
0
+
0
+
0
+
+
0
+
0
+
0
0
+
+
+
+
0
0
rr
9
0
0
+
0
+
0
+
0
+
+
0
0
+
+
0
+
0
+
0
0
rr
10
0
0
+
0
+
0
+
0
+
0
+
0
+
+
0
+
+
+
0
0
R1wR1
11
+
+
0
0
+
0
+
+
0
+
+
0
+
0
+
0
+
0
0
2+
NT
Patient’s Cell
Auto control
PC
0
0