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The page below is a sample from the LabCE course ABO Typing Discrepancies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example: Unexpected Reaction in Reverse Typing

Forward (Cell) Grouping
Reverse (Serum) Grouping
Anti-A
Anti-B
Anti-A,B
A1 Cells
B Cells
4+
0
4+
2+
4+
Most probable ABO type: Group A
Possible causes: A2 patient with an anti-A1, abnormal serum proteins resulting in rouleaux formation, a room temperature reactive alloantibody, or a cold autoantibody.
Some strategies for resolution:
  • Perform an antibody screen
  • Test patient cells with anti-A1 lectin
  • Perform saline replacement techniques