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The page below is a sample from the LabCE course ABO Typing Discrepancies. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Example: Weak/Missing Reaction in the Reverse Typing

Forward (Cell) Grouping
Reverse (Serum) Grouping
Anti-A
Anti-B
Anti-A,B
A1 Cells
B Cells
4+
0
4+
0
0
Most probable ABO type: Group A
Possible causes: Immunocompromised, age-related, transplantation, dilution, technical/procedural error
Some strategies for resolution: Repeat testing with careful attention to accuracy or repeat reverse grouping with extended incubation at or below room temperature. Weak ABO reactions can often be potentiated using extended incubation at or below room temperature.