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The page below is a sample from the LabCE course Microbial Identification Using MALDI-TOF MS. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Alatoom AA, Cunningham SA, Ihde SM, Mandrekar J, Patel R. Comparison of direct colony method versus extraction method for identification of gram-positive cocci by use of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011;49(8):2868-73. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147777/. Accessed September 3, 2019.
Besséde E, Angla-Gre M, Delagarde Y, Sep Hieng S, Ménard A, Mégraud F. Matrix-assisted laser-desorption/ionization biotyper: experience in the routine of a University hospital. Clin Microbiol Infect. 2011;17(4):533-538. Available at: http://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)63269-9/fulltext. Accessed September 3, 2019.
Buchan BW, Riebe KM, Ledeboer NA. Comparison of the MALDI Biotyper System Using Sepsityper Specimen Processing to Routine Microbiological Methods for Identification of Bacteria from Positive Blood Culture Bottles. J Clin Microbiol. 2012;50:346-352. Available at: http://jcm.asm.org/content/50/2/346.full. Accessed September 3, 2019.
Chen JHK, Ho PL, Kwan GSW, She KKK, Siu GKH, Cheng VCC, Yuen KY, Yam WC. Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. J Clin Microbiol. 2013;51(6):1733-9. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3716069/. Accessed September 3, 2019.
Chen JHK, She KKK, Wong OY, Teng JLL, Yam WC, Lau SKP, Woo PC, Cheng VCC, Yuen KY. Use of MALDI Biotyper plus ClinProTools mass spectra analysis for correct identification of Streptococcus pneumoniae and Streptococcus mitis/oralis. J Clin Pathol. 2015;68(8):652-656. Available at: http://jcp.bmj.com/content/68/8/652. Accessed September 3, 2019.
Clinical and Laboratory Standards Institute (CLSI). Methods for the Identification of Cultured Microorganisms Using Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry. First ed. CLSI document M58. Wayne, PA: CLSI; 2017.
Croxatto A, Prod'hom G, Greub G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology. FEMS Microbiol Rev. 2012;36(2):380-407. Available at: https://academic.oup.com/femsre/article/36/2/380/565595. Accessed September 3, 2019.
Desai AP, Stanley T, Atuan M, McKey J, Lipuma JJ, Rogers BE, Jerris, R. Use of matrix assisted laser desorption ionisation-time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients. J Clin Pathol. 2012;65(9):835-838. Available at: http://jcp.bmj.com/content/65/9/835.long. Accessed September 3, 2019.
Doern, CD. Charting Uncharted Territory: a Review of the Verification and Implementation Process for Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) for Organism Identification. Clinical Microbiology Newsletter. 2013;35(9):69–78. Available at: https://www.deepdyve.com/lp/elsevier/charting-uncharted-territory-a-review-of-the-verification-and-0FiCjgDmyh. Accessed September 3, 2019.
Fiori B, D'Inzeo T, Di Florio V, De Maio F, De Angelis G, Giaquinto A, Campana L, Tanzarella E, Tumbarello M, Antonelli M, Sanguinetti M, Spanu T. Performance of two resin-containing blood culture media in detection of bloodstream infections and in direct matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) broth assays for isolate identification: clinical comparison of the BacT/Alert Plus and Bactec Plus systems. J Clin Microbiol. 2014;52(10):3558-3567. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4187791/. Accessed September 3, 2019.
Fothergill A, Kasinathan V, Hyman J, Walsh J, Drake T, Wang YFW. Rapid identification of bacteria and yeasts from positive-blood-culture bottles by using a lysis-filtration method and matrix-assisted laser desorption ionization-time of flight mass spectrum analysis with the SARAMIS database. J Clin Microbio. 2013;51(3):805–809. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592086/. Accessed September 3, 2019.
Gaillot O, Blondiaux N, Loïez C, Wallet F, Lemaître N, Herwegh S, Courcol RJ. Cost-effectiveness of switch to matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine bacterial identification. J Clin Microbiol. 2011;49(12):4412. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232935/. Accessed September 3, 2019.
Machen A, Drake T, Wang YFW. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system. PloS One. 2014;9(2:e87870. Available at: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0087870. Accessed September 3, 2019.
Manji R, Bythrow M, Branda JA, Burnham CA, Ferraro MJ, Garner OB, Jennemann R, Lewinski MA, Mochon AB, Procop GW, Richter SS, Rychert JA, Sercia L, Westblade LF, Ginocchio CC. Multi-center evaluation of the VITEK® MS system for mass spectrometric identification of non-Enterobacteriaceae Gram-negative bacilli. Eur J Clin Microbiol Infect Dis. 2014;33(3):337-346.
McElvania Tekippe E, Shuey S, Winkler DW, Butler MA, Burnham CAD. Optimizing identification of clinically relevant Gram-positive organisms by use of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry system. J Clin Microbiol. 2013;51(5):1421–1427. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647943/. Accessed September 3, 2019.
Patel, R. Matrix-assisted laser desorption ionization-time of flight mass spectrometry in clinical microbiology.Clin Infect Dis. 2013;57(4):564–72.
Perez KK, Olsen RJ, Musick WL, Cernoch PL, Davis JR, Peterson LE, Musser J M. Integrating rapid diagnostics and antimicrobial stewardship improves outcomes in patients with antibiotic-resistant Gram-negative bacteremia. J Infect. 2014;69(3):216-225. Available at: http://www.journalofinfection.com/article/S0163-4453(14)00134-0/fulltext. Accessed September 3, 2019.
Tan K E, Ellis BC, Lee R, Stamper PD, Zhang SX, Carroll KC. Prospective evaluation of a matrix-assisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness. J Clin Microbiol. 2012;50(10):3301–3308. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457442/. Accessed September 3, 2019.