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The page below is a sample from the LabCE course Autism Spectrum Disorders: Genetic Testing. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Genetic Testing for ASD: Chromosomal Microarray Analysis (CMA), continued

The diagram depicts a typical CMA method. Initially, the reference DNA (in red) is placed on a microslide and the test DNA (in green) from the patient is extracted, purified, denatured (strand separation), and then hybridized to the complimentary DNA probes on the slide. The slide containing the differentially-labeled DNA (patient and reference) produces signal outputs that are then scanned and analyzed by a computer-based analyzer. Normal pieces of DNA hybridized equally will produce a yellow signal, whereas extra pieces of DNA (duplication) will yield a green signal and missing pieces of DNA (deletions) produce a red signal.