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The page below is a sample from the LabCE course Linear Regression Analysis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Prediction Using the Resulting Equation

Once the parameters have been calculated, the resulting equation can be used to make predictions about a value of y given a value of x, provided that the x value is in the same range of x-values that were used to derive the equation.
In this example, we have calculated a=0 and b= 0.002
If x = 350 mg/dL, what is the expected value of y? To find the answer, substitute the known value of x into the equation. y = 0 + (0.002)(x)   When the concentration is 350 mg/dL, we expect the absorbance to be about 0.7.