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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Change Early, Change Often!

Once of the easiest ways to keep your H&E slides looking good is to simply ensure frequent reagent changes. Most manufacturers have some information about the number of slides that their reagents may tolerate before they must be changed. While this data is a guideline, see what makes sense for your laboratory. If changing reagents daily is a better fit, then change daily.