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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Charged Slides Used with Adhesives

You have both charged (plus) slides and adhesives, so using them together should make the tissue stick even better, right? Wrong! The nature of both methods is to attract ions from the tissue. When used together, the properties of both cancel each other out, essentially changing the positively changed slide into a plain glass slide.