Home Products Most Popular Contact
No items in your cart.
The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

Learn more about Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain (online CE course) »
How to Subscribe
Histology CE Package$65 Add to cart
Individual course$20 Add to cart

The hematoxylin stain in this section of colon appears to be taking over
the section. Even the mucin in the goblet cells are stained blue.

Acid Extends Hematoxylin Life: Why do I Need it for Differentiation?

A mild acid solution is important for clearing the nuclei and reducing overall background staining on the slide. When this step is inadequate, nuclei appear overstained and a purple haze will be noted grossly on the slide. Microscopically, mucin will be stained purple and may obscure details necessary for diagnosis.