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Interpretation of ABO Group

We can use the forward type together with the reverse type to interpret the ABO group. The expected reaction are as follows:

Red Cells Tested With Known Antisera

Serum Tested With Known Red Cells

Interpretation of ABO Group

Anti-A

Anti-B

Anti-A,B

A1 Cells

B Cells

3-4+

0

3-4+

0

3-4+

A

0

3-4+

3-4+

3-4+

0

B

0

0

0

3-4+

3-4+

O

3-4+

3-4+

3-4+

0

0

AB

+ = agglutination (graded 1+ to 4+)
0 = no agglutination or hemolysis