Again, it is important that the proper balance of the dyes is achieved. Overstaining with hematoxylin can give the illusion of under stained eosin, just as overstaining with eosin can cause the hematoxylin to appear lighter than it actually is. So, when optimizing the stain, make sure to only edit the time of one of the components. This technique will help eliminate the need to spend additional time adjusting the stain.
