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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Protocol is Too Long

Oftentimes, simply decreasing the time in hematoxylin and/or eosin can improve the coloration. Shortening steps in increments of 30 seconds for hematoxylin and 15 seconds for eosin can gradually decrease the intensity to the desired coloration.