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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Good staining - placenta

Ease of Use and Reproducibility

The staining protocol for H&E follows a basic protocol:
  • Dewaxing
  • Dehydration
  • Hematoxylin
  • Differentiation
  • Bluing
  • Eosin
  • Dehydration
  • Clearing
  • Coverslipping
The format is easily reproduced and the reagent resilient enough to allow for large numbers of slides to be stained consistently before reagents need to be changed.