In isoelectric focusing, the basis of separation of solutes is different than the other types of electrophoresis. Which statement below correctly describes this feature of isoelectric focusing?

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	Ampholytes create a pH gradient on the gel and enable separation of solutes based upon their pIs
	Sodium dodecyl sulfate (SDS) creates a pH gradient and enables separation of solutes by their pIs
	Ampholytes break the solutes into smaller size particles and increase the speed of the separation
	Isoelectric focusing separates the sample solutes by two distinct electrophoresis methods