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The page below is a sample from the LabCE course Electrophoresis. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Routine Electrophoresis

Routine electrophoresis (also known as zone electrophoresis) is performed on a rectangular slab gel. Several patient specimens and control(s) can be placed on one gel and solutes separated in one run.
Routine electrophoresis is mostly used for separation of proteins. A serum sample with normal plasma proteins yields five zones or bands of separated proteins: albumin, alpha-1-globulins, alpha-2-globulins, beta-globulins, and gamma-globulins. CSF and urine proteins can also be separated with routine electrophoresis. Other applications include separation of isoenzymes, hemoglobins, and fragments of DNA and RNA.
A manual agarose gel electrophoresis of eight serum samples is pictured below. After electrophoresis, the gel was stained with Ponceau S.

Agarose Gel Electrophoresis