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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Regressive Staining

Regressive staining follows the hematoxylin step with a differentiator specifically designed to remove excess stain from the nuclei. Strong or weak acids may be used; however, weak acids tend to give more control over the amount of dye removed.