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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Progressive and Modified Progressive Staining

Progressive staining occurs when the hematoxylin is added to the tissue without being followed by a differentiator to remove excess dye. Modified progressive staining occurs when a mild differentiator is used not to remove excess stain from the nuclei, but rather removes background staining. So, the key to whether a stain is progressive is whether the nuclear stain is altered, not whether there is a differentiator present.