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The page below is a sample from the LabCE course Troubleshooting Guidance for Hematoxylin and Eosin (H&E) Stain. Access the complete course and earn ASCLS P.A.C.E.-approved continuing education credits by subscribing online.

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Elastic fibers of aorta section stained with Verhoff-Van Gieson

The Iron Hematoxylins

These hematoxylins use iron salts as a mordant and are typically used in special stains. This is because they can demonstrate more tissue structures than alum hematoxylins, such as myelin and elastin fibers. One of the best know is Weigert's, which is used in the Verhoff-Van Gieson stain, shown in the image.