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HIV: Structure, Replication, and Detection (Online CE Course)

(based on 527 customer ratings)

Author: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB)
Reviewer: Barbara Cebulski, MS, MLS(ASCP)CM

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1.5 hour(s)
Course number 578-027-16, approved through 4/30/2018
Florida Board of Clinical Laboratory Personnel Credit Hours - General (Serology/Immunology): 1.5 hour(s)
Course number 20-547705, approved through 9/1/2018


  • Explain the basic structure of HIV.
  • Discuss the process by which HIV replicates.
  • Discuss commonly used laboratory methods for detection of HIV infection.
  • Compare the advantages and disadvantages of commonly used laboratory test methods for detection of HIV infection.

Customer Ratings

(based on 527 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • Structure of the Human Immunodeficiency Virus
      • Basic Structural Components
      • HIV Envelope
      • HIV Capsid
      • Viral proteins are identified as either glycoprotein (gp) or protein (p). What is indicated by the number that follows the "gp" or "p" designation, fo...
  • Replication
      • Proteins Involved in Adsorption
      • Adsorption
      • Adsorption, continued
      • Penetration and Infection
      • DNA Conversion from RNA
      • HIV is a Retrovirus
      • Infection of the Host Cell
      • Infection of the Host Cell, continued
      • Spread of Infection
      • Spread of Infection, continued
      • Spread of Infection, continued
      • Destruction of the Host Cell
      • What is the protein that coats the genome in the core of the HIV-1 virion?
      • The enzyme found in the core of the HIV virion that is utilized to convert viral RNA into DNA is called reverse transcriptase.
  • HIV Virus Classifications
      • Mutations
      • Most HIV-1 infections are classified as which one of the following groups?
  • Progression of HIV Infection
      • Stages of HIV Infection
      • Which stage of HIV infection is characterized by a decline in the CD4 T-lymphocyte count to less than 200 cells/µL?
  • Laboratory Methods to Detect and Monitor HIV Infection
  • References
      • References

Additional Information

 Level of instruction: Intermediate

Intended Audience: Medical laboratory technologists and technicians and other health care personnel who have an interest in this subject matter. This course is also appropriate for clinical laboratory science students and pathology residents.
Author information: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB) is Laboratory Director & Director of Laboratory Operations, Quest Diagnostics-Valdosta RRL. Dr. Pendergraph is the laboratory director for Quest Diagnostics in Valdosta, GA and Miller County Hospital in Colquitt, GA. Dr. Pendergraph received his MSPH from the University of Kentucky in Lexington, his PhD in medical parasitology and mycology from the University of  North Carolina in Chapel Hill and his law degree with a concentration in health care law from Concord Law School, Kaplan University. He also did a Fellowship in Tropical Medicine at Louisiana State University School of Medicine. He is the author of a textbook in phlebotomy, a number of scientific articles, plus internet training programs. Dr. Pendergraph serves on the advisory committee for medical technology program at Thomas University. He is licensed as a laboratory director in the States of Georgia and Florida.
Reviewer: Barbara Cebulski, MS, MLS(ASCP)CM is a program director for MediaLab, Inc., responsible for the development of continuing education and competency assessment courses for laboratory professionals. Prior to 2007, Ms. Cebulski was an Inspection Specialist in the Laboratory Accreditation Program for the College of American Pathologists (CAP). During this time, she also presented on behalf of CAP at Inspector Training seminars and Point-of-Care Group conferences. In her career as a medical technologist, she has held management and supervisory positions. Barbara holds a Masters degree in Instructional Design and Technology from Georgia State University.

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