Department of Transportation (DOT) Federally Regulated Urine Specimen Collection Training (Online CE Course)

(based on 82 customer ratings)

Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB)

Department of Transportation (DOT) regulated urine specimen collections are an important part of employment and workplace screenings. As a collector, you'll perform DOT urine specimen collections for drug and alcohol testing, return-to-duty testing, and more.

In order to perform DOT-regulated urine specimen collections, you'll need to complete qualification training. MediaLab's online course in Department of Transportation (DOT) Regulated Urine Specimen Collection satisfies the requirements for qualification training as outlined in Federal Regulations, 49 CFR Part 40.

Important: This course requires you to find a qualified observer to observe five required mock collections. This course is normally used by hospitals, laboratories, and physician offices that already have a collector on staff who can be an observer. If you are a private individual, this course may not be appropriate for you. MediaLab & LabCE do not provide observers or maintain a list of observers for you to contact.

What does this course include?

This course includes three components:
  1. Online reading material covering DOT rules and procedures (about 1 - 2 hours to cmplete)
  2. A short exam covering the reading material (about 10 - 15 minutes to complete)
  3. A checklist to record completion of five mock collections (time varies)

How to become qualified to perform DOT urine collections

There are two steps to becoming qualified for DOT urine collection:
  1. Complete a qualification training course, like this online course from MediaLab.
  2. Complete five mock collections under various scenarios in the presence of a qualified observer.

MediaLab's online DOT urine collection training course includes an integrated checklist feature to document completion of these required mock collections, but MediaLab does not provide observers for the mock collections. You will need to locate a qualified collector locally who can attest that you have completed the mock collections.

Who can observe the mock collections for DOT qualification training?

A qualified collector must perform the observations. A qualified collector must either (a) regularly perform DOT-regulated urine specimen collections, (b) regularly observe collections for DOT training requirement, or (c) have completed a "train the trainer" course. Observations must be conducted in person by a qualified collector and cannot be performed online or remotely.

How do I find an observer?

This course is normally used by hospitals, laboratories, and physician offices that already have a collector on staff who can be an observer. If you are a private individual without access to an observer, this course may not be appropriate for you.

How does an observer report completion of the mock collections to MediaLab?

An observer will complete your online checklist and provide his / her contact information. The completed checklist, including the observer's contact information, becomes part of your certification documentation. MediaLab does not validate or audit completed observations. An individual or company that wants to validate your credentials may need to contact your observer.

Am I DOT-certified after completing this course?

Once you have completed the qualification training from MediaLab and documented that you have completed the five required mock collections, then you are qualified to perform DOT urine specimen collections. You will receive a certificate from MediaLab attesting that you have completed qualification training, and you will be able to print out documentation that you have completed the mock collections. The DOT does not issue certificates, licenses, or collector numbers, so you will not receive any materials from the DOT itself.

How long is the certification good for?

After completing the qualification training and five mock collections, you must complete refreshing training within five years. You are responsible for keeping up with changes to DOT regulations and making sure that you comply with any changes.

Is this course acceptable for refresher training under DOT Rule 49 CFR Part 40 Section 40.33 Subpart C?

Yes. You must complete both the qualificiation training (reading, video, exam) and complete the five mock collections in order to satisfy the requirements for refresher training.

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Continuing Education Credits

P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2.5 hour(s)
Course number 578-011-16, approved through 3/31/2018
Course number 20-547617, approved through 9/1/2018


  • Describe the three types of urine specimen collections.
  • Identify the different types of custody and control forms and explain how to complete them properly.
  • Describe the correct sequence of a proper drug screen collection.
  • Identify and explain how to hande unusual collection situations.

Customer Ratings

(based on 82 customer ratings)

Course Outline

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Additional Information

Intended Audience: Personnel who will be conducting DOT urine drug screen collections.

Level of Instruction: Basic. The course follows the "Urine Specimen Collection Guidelines" for the U.S. Department of Transportation Workplace Drug Testing Programs (49 CFR Part 40).

Author Credentials: Garland E. Pendergraph, PhD, JD, MLS(ASCP)SM, HCLD/CC(ABB) is Laboratory Director & Director of Laboratory Operations, Quest Diagnostics-Valdosta RRL. Dr. Pendergraph is the laboratory director for Quest Diagnostics in Valdosta, GA and Miller County Hospital in Colquitt, GA. Dr. Pendergraph received his MSPH from the University of Kentucky in Lexington, his PhD in medical parasitology and mycology from the University of  North Carolina in Chapel Hill and his law degree with a concentration in health care law from Concord Law School, Kaplan University. He also did a Fellowship in Tropical Medicine at Louisiana State University School of Medicine. He is the author of a textbook in phlebotomy, a number of scientific articles, plus internet training programs. Dr. Pendergraph serves on the advisory committee for medical technology program at Thomas University. He is licensed as a laboratory director in the States of Georgia and Florida.


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Donor initials the tamper-evident seal

Handing over a urine sample cup

Materials needed for specimen collection.

White CCF and samples are sealed in a bag