Detecting Metallo-Beta-Lactamases and Colistin Resistance in Gram Negative Bacilli (by CACMLE) (Online CE Course)

(based on 22 customer ratings)

Paul Schreckenberger, PhD, D(ABMM)

Developed from a teleconference, the goal of this self-study course is to use a structured, yet self-paced format to provide a review of metallo-beta-lactamase and colistin resistance mechanisms, and the phenotypic methods used to identify these mechanisms in the Gram Negative bacilli.

Presented in an easy-to-use PowerPoint slide format with corresponding audio, it’s just like being at a live presentation!

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  • Recognize pitfalls of susceptibility testing for detection of antibiotic resistance
  • Identify best methods for detecting metallo-beta-lactamases and colistin resistance.
  • Add appropriate interpretative comments to susceptibility reports to aid in physician understanding

Customer Ratings

(based on 22 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • PowerPoint Slides
      • Detecting Metallo-Beta-Lactamase and Colistin Resistance in Gram Negative Bacilli
      • Course Goal
      • Course Overview
      • Author
      • Introduction
      • Learning Objectives
      • Case 1
      • Case 1-MicroScan Report
      • Case 1- MicroScan Panel, cont.
      • Case 1 - ID Consult
      • Case 1 - 12 Disk Test Result
      • Case 1 - Modified Hodge Test
      • MBL E Test, Colistin, Tigecycline Results
      • Case 1 - Sensititre Results: E. cloacae
      • The Answer is....
      • Carbapenemases in the U.S.
      • Metallo-beta-lactamases belong to which molecular class of carbapenemases:
      • MicroScan Panel
      • IMI/NMC-A Enzymes
      • You have isolated an Enterobacter cloacae from a positive blood culture. AST gives the following results: ampicillin is resistant, all cephalosporins ...
      • Case 2
      • Case 2, cont.
      • Case 2 - MicroScan Report
      • Case 2 - MicroScan Report, cont.
      • Case 2 - 12 Disk Test Results
      • Case 2- MBL ETest, Colistin, Tigecycline
      • Case 2 - Sensititre Results: K. pneumoniae
      • Polymyxin Antibiotics - Mechanism of Action
      • The target for polymyxin antibiotics is the cell membrane.
      • Polymyxin Antibiotics - Development of Resistance
      • Polymyxin Antibiotics - Pharmacokinetics
      • Polymyxin Antibiotics - Spectrum of Activity
      • Polymyxin Antibiotics - Susceptibility Testing
      • Assessment of Colistin and Polymyxin B AST Methods - Breakpoints
      • Acinetobacter spp: Colistin MIC vs. Disk
      • Acinetobacter spp: Polymyxin B MIC vs. Disk
      • Assessment of Colistin and Polymyxin B AST Methods - Acinetobacter spp.
      • • For Acinetobacter spp. - disk diffusion methods are able to reliably detect colistin and polymyxin resistance (MIC >4 mg/ml)
      • Ps. aeruginosa: Colistin MIC vs. Disk
      • Ps. aeruginosa: Polymyxin B MIC vs. Disk
      • Assessment of Colistin and Polyxin B AST Methods - Ps. aeruginosa
      • Assessment of Colistin and Polymyxin B AST Methods - Error Rates
      • Assessment of Colistin and Polymyxin B AST Methods - Conclusions
      • Colistin/Polymyxin B: Breakpoints for Ps. aeruginosa
      • Colistin/Polymyxin B: Breakpoints for Acinetobacter
      • Testing Caveats
      • Testing Caveats, cont.
      • When testing for colistin resistance, polymyxin should be the preferred choice as a representative of the polymyxin antibiotics.
      • Colistin Resistant KPC
      • A colistin and tigecycline resistant KPC may be treated with:
      • Colistin Resistant KPC, cont.
      • Colistin Resistant KPC, cont.
      • Conclusion
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