Bacterial Biofilms: What are they and are they important? (by CACMLE) (Online CE Course)

(based on 31 customer ratings)

Rita Heuertz, PhD, MT(ASCP)

Biofilm formation is recognized as causing or exacerbating numerous chronic infections including periodontitis, device-related infections, cystic fibrosis pneumonia, chronic urinary tract infections, recurrent tonsillitis, chronic rhinosinusitis and chronic wound infections. Of grave concern, presence of biofilm is associated with increased bacterial resistance to antimicrobic therapy and resultant treatment failure. The role of the clinical laboratory in diagnosis of biofilm-associated infection is in its initial stages of addressing this problem. This self-study serves as an update on biofilms and their identification. This course was adapted from a presentation given by the author for the CACMLE “2012 Grand Rounds in Clinical Laboratory Microbiology and Infectious Disease” Teleconference Series. The audio component of this course was modified from this presentation and divided into frame specific narrations.

In a structured, yet self-paced format, the goals of this course are to provide participants with:

  • fundamental terms and concepts relevant to theory and techniques associated with bacterial biofilms.
  • information obtained from literature review of recent PubMed publications on biofilms.
  • an appreciation of the importance of biofilm-associated infections.
  • information on biofilm assay development.

Presented in an easy-to-use PowerPoint slide format with corresponding audio, it’s just like being at a live presentation!

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  • State the definition of biofilm.
  • Describe bacteria that form biofilms.
  • Discuss the association of biofilm with increased resistance to antimicrobic agents.
  • Assess and identify biofilm assays applicable to the clinical laboratory.

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(based on 31 customer ratings)

Course Outline

Click on the links below to preview selected pages from this course.
  • PowerPoint Slides
      • Bacterial Biofilms: What are they and are they important?
      • Goals for Course
      • Course Overview
      • Course Author
      • Introduction
      • Learning Objectives
      • Biofilm Definition
      • Definition
      • Description of Bacteria That Form Biofilms
      • Biofilm Growth Characteristics
      • Antimicrobic Resistance in a Biofilm Mass
      • Biofilm-associated microbial infection is:
      • Medical Importance of Biofilms
      • NIH Statistics on Biofilm Infections
      • Biofilm Causes Chronic Infection
      • Biofilms are important because:
      • Biofilm Causes Interventional Device Infections
      • One of the first biofilm-associated infections identified and therefore best described currently is:
      • Microbes Commonly Associated with Biofilms on Indwelling Medical Devices
      • Staph Biofilm on a Medical Device
      • Staph Biofilm on a Prosthetic Device
      • Biofilm in Living Tissue
      • Biofilm Definition Revisited
      • Diagram of a Medical Biofilm
      • Immune Response to Biofilm
      • Chronic Pressure Ulcer Infection: Dense PMN Infiltration
      • Immune Response to Biofilm
      • Medical Importance of Biofilms
      • Diagnostic Problems
      • Diagnostic Problems for Physicians
      • Diagnostic Problems for the Lab
      • Clinical wound specimens showing no growth in culture despite the presence of clinical symptoms or patient complaints of pain:
      • Methods for Biofilm Identification
      • Laboratory Tests for Biofilm
      • Qualitative Stain-Based Assay
      • Quantitative Stain-Based Assay
      • Qualitative & Quantitative Stain-Based Biofilm Assays
      • Clinical Isolates of MRSA with High Levels of Biofilm Production and Antimicrobic Resistance
      • Clinical Isolates of Ps. aeruginosa with High Levels of Biofilm Production and Antimicrobic Resistance
      • Assessment of Tube-Based Assays
      • Microplate Quantitative Stain-Based Assay
      • Microplate Quantitative Stain-Based Assay, cont.
      • Microplate Peg-Lid Quantitative Stain-Based Biofilm Assay
      • Microplate Peg-Lid Quantitative Stain-Based Biofilm Assay, cont.
      • Solubilization of Stain in Stain-Based Biofilm Assays
      • Treatment Strategies
      • Wound Care Strategies
      • Current Treatment Strategies
      • Hope for the Future
      • Research: Hope for the Future
      • Lab: Hope for the Future
      • Physician: Hope for the Future
      • Physician: Hope for the Future, cont.
      • Patient: Hope for the Future
      • Conclusion
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Individual course$25 Add to cart

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