Human Papillomavirus (HPV) and Molecular Diagnostic Testing (Online CE Course)
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Human papillomavirus (HPV) is a frequently occurring viral infection that is sexually transmitted. HPV infections often present with mild symptoms or are asymptomatic and do not always progress to a disease state. However, some HPV infections can cause cervical or other anogenital carcinomas. If HPV screening is done regularly, most cervical cancers can be prevented. In fact, cervical cancer is one of the most successfully treated cancers. Management of HPV has changed dramatically with the introduction of DNA methods for diagnosis of HPV infections, and vaccines for the prevention of HPV infections. This course is intended to increase your knowledge about HPV and to acquaint you with molecular methods that are currently available for detection of HPV DNA in cervical cells.
Continuing Education Credits
- P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
- Florida Board of Clinical Laboratory Science CE - Molecular Pathology: 2 hour(s)
- Describe human papillomavirus (HPV) structure, genome, encoded proteins and its pathology as a sexually transmitted infection(STI).
- Differentiate various HPV viral types while comparing high risk and low risk types.
- Explain the relationship between HPV and cervical cancer, carcinogenesis of cervical cancer including a description of tumor suppressor genes, oncogenes and the virulence of HPV types 16 and 18.
- Compare and contrast molecular assays for high risk HPV types: High Risk HPV DNA Hybrid Capture 2 and Cervista HPV assays.
- Compare and contrast vaccines to prevent HPV infections: Gardasil and Cervarix.
- Evaluate appropriate and inappropriate utilization of molecular testing for HPV DNA in cervical cancer screening.
- Discuss basic molecular diagnostic processes: denaturation, hybridization, and types of amplification methods.
- Explain basic molecular biology concepts: composition of nucleic acids and complementary base pairing.
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- Human Papillomavirus (HPV)
- Organizations and Agencies
- HPV as a Virus
- HPV Structure
- HPV Viral Types
- Low-Risk and High-Risk HPV Types
- HPV Genome and Proteins
- Which of the following HPV types are high-risk HPV types:
- Select the statement that correctly describes the HPV genome and its endoded proteins.
- HPV as a Sexually Transmitted Infection (STI)
- HPV as a Sexually Transmitted Infection
- Clinical Diseases Caused by HPV STI Types
- Most genital condolymas and cervical lesions caused by HPV resolve as a result of the immune response in the infected individual.
- HPV Types and Cervical Cancer
- HPV and Cervical Cancer
- Immune Mediated Regression
- Life Cycle of Human Papillomavirus
- Additional Cervical Cancer Factors
- Carcinogenesis of Cervical Cancer
- Review: Tumor Suppressor Genes, Proto-Oncogenes and Oncogenes
- Carcinogenesis of Cervical Cancer Continued
- HPV infection can linger for years in cervical cells and eventually convert normal cells into malignant cells.
- Papanicolaou (Pap) Test and Cervical Cytology Testing
- Pap Smear
- Pap Smear Images
- Pap Smear and Cervical Cancer
- Pap Smear Limitations
- HPV Molecular Diagnostics
- Molecular Detection of HPV
- Molecular Detection of HPV in Cervical Cytology Specimens
- Review: Nucleic Acids and Molecular Diagnostic Testing
- Nucleic Acids: DNA and RNA
- Base Pairing in Nucleic Acids
- Comparison of DNA and RNA
- Molecular Testing Basics
- Which nitrogen base would bind with a guanine nucleotide in forming double-stranded DNA?
- Review the descriptions of molecular testing techniques and select the correct statements.
- Types of Molecular HPV Testing
- Molecular Methods for HR-HPV Testing
- High-Risk HPV Hybrid Capture (HC) 2 DNA Test
- Step 1 High-Risk HPV HC2 DNA Assay
- Step 2 High-Risk HPV HC2 DNA Assay
- Step 3 High-Risk HPV HC2 DNA Assay
- Step 4 High-Risk HPV HC2 DNA Assay
- Step 5 High-Risk HPV HC2 DNA Assay
- Cervista HPV DNA
- Cervista HPV DNA Oligonucleotides
- Cervista HPV DNA Primary Reaction
- Cervista HPV DNA Primary Reaction Continued
- Cervista HPV DNA Secondary Reaction
- Comparison of HPV DNA Testing
- Which HR-HPV type is screened for in the Cervista HPV DNA test and not in the High-Risk HPV HC2 DNA test?
- Twenty-five samples of isolated DNA from liquid cervical cytology specimens, one negative control, and one positive control were assayed with the High...
- HPV DNA Test Utilization
- Recommended Use of HPV DNA Testing in Cervical Cancer Screening
- Inappropriate Use of HPV DNA Testing
- Case A Michelle
- What is the recommended HPV DNA testing for Michelle, a 19-year-old female with ASC-US Pap smear report?
- Case B Darlene
- HR-HPV DNA testing was appropriately ordered on Darlene.
- Case C Josephine
- Josephine calls her physican office and questions why HPV DNA testing was done along with her Pap test?
What is the best response to her question?
- Case D Debra
- Debra's cervical cancer screening report was Pap test negative but her HR-HPV DNA was positive.
It was appropriate for her physician to schedule her ...
- Vaccination for HPV
- HPV Vaccines
- HPV Vaccines; Gardasil and Cervarix
- What would the vaccination recommendations be for the four women in the case studies presuming that none had previously received immunization?
Level of instruction: Intermediate
Intended Audience: Clinical laboratory technologists and technicians, and other health care personnel who have an interest in this subject matter. This course is also appropriate for medical laboratory science students, pathology residents, and practicing pathologists.
Author information: Mary Ellen Koenn, MS, MT(ASCP), CLS(NCA) is an associate professor at West Virginia University School of Medicine in the Medical Technology Program. During her career as a medical technologist and educator, she has been a laboratory supervisor and manager and has held several teaching positions. She is active in the American Society for Clinical Laboratory Science (ASCLS), resulting in a nomination as ASCLS Member of the Year in 2006. She is also a member of the American Association for Clinical Chemistry. She is the author of numerous articles for laboratory publications and textbook chapters and is a frequent presenter at laboratory seminars and workshops. Ms. Koenn holds a Master of Science degree in Medical Technology.
Reviewer information: Karen S. Long, MS, MT(ASCP), CLS(NCA) is an Associate Professor Emerita at West Virginia University School of Medicine. During her career in the field of laboratory medicine, her teaching and laboratory responsibilities have been in the disciplines of Microbiology, Mycology, and Immunology. She is a frequent presenter of workshops and seminars on topics in Microbiology. Ms. Long holds a Master of Science degree in Medical Technology Education.
Course Description: This course discusses the various types of human papillomavirus and their association with cervical cancer. Molecular methods of detection are discussed, beginning with a basic review of nucleic acid amplification methods and hybridization methods. Two FDA-approved methods for molecular HPV testing are briefly explained. The course also presents four case studies to demonstrate the correct and incorrect utilization of HPV molecular methods.
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