Confirmatory and Secondary Urinalysis Screening Tests (Online CE Course)
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Authors: Carol Campshure, M.S., MT(ASCP), Betty Smith, MT(ASCP) and Beverly Schieltz, M.S., MT(ASCP)
Reviewer: Barbara Cebulski, MS, MLS(ASCP)CM
Urine reagent strip testing is usually a reliable method when screening specimens for glucose, protein, ketones, and bilirubin. However, it occasionally may be necessary to use a secondary method to confirm a result or to test for an analyte that may not be included in the reagent strip method. This course will help a tech to determine when a secondary or confirmatory urine screening method may be needed to ensure accurate patient test results. This course is appropriate for laboratory professionals and for clinical laboratory science students.
Continuing Education Credits
- P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 1 hour(s)
- Florida Board of Clinical Laboratory Science CE - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)
Objectives
- Determine when an alternative procedure should be used to confirm a urine reagent strip result or to detect substances that are not within the sensitivity or specificity of the urine reagent strip test.
- Discuss the appropriate specimen and reagent handling procedures.
- Identify interfering substances that can affect confirmatory and secondary tests.
- Recognize the limitations of each procedure.
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Course Outline
- Why are confirmatory and secondary tests performed?
- Reasons for Performing Confirmatory or Secondary Macroscopic Urine Tests
- Detection of Urinary Sugars and Reducing Substances
- Urine Glucose
- Reducing Sugars
- Testing Methods for Urine Glucose and Other Reducing Substances
- Specimen Processing for Urine Sugar Testing
- Testing for Reducing Substances Other Than Glucose
- Copper Reduction Reaction
- What is the test principle of the Clinitest® reaction?
- What is indicated if a sugar result on a reagent dipstick is negative and a Clinitest® result on the same specimen is positive?
- Detection of Urinary Protein
- Diseases Associated with Proteinuria
- Screening and Secondary Tests for Protein
- SSA Test Procedure and Results Interpretation
- SSA Procedural Notes and Test Limitations
- Microalbumin Test
- A routine reagent strip test for protein is based on the principle of:
- Persons with type 1 or type 2 diabetes should periodically have their urine monitored for which of the following to avert renal complications related ...
- Which of the following conditions may require the use of the sulfosalicylic acid (SSA) test to detect protein as part of a routine urinalysis?
- Albumin is the main serum protein found in normal urine.
- Detection of Urinary Ketone Bodies
- Urinary Ketones
- Causes of Ketonuria
- Detection of Ketone Bodies
- Acetest®
- Detection of Urinary Bilirubin
- Urine Bilirubin
- Bilirubinuria
- Confirmation of Urine Bilirubin Result
- Diazo Tablet Test
- Limitations of the Procedure
- What is the basis of both the reagent strip test and Ictotest® for detection of bilirubin?
- Unconjugated bilirubin is conjugated in the liver with which of the following substances to become water soluble?
Additional Information
Level of Instruction: Basic
Intended Audience: Medical laboratory scientists, Medical technologists, and medical technicians seeking review, cross-training, or continuing education opportunities; clinical laboratory science students, medical students and pathology residents.
Course Description: This illustrated course cover the basics of confirmatory and secondary urinalysis testing, including preparation, collection, reagents, limitations, and quality control. Images of test reactions and interactive questions throughout the course reinforce concepts and procedures.
About the Course: This is a revised course that was originally part of a series of courses adapted for the web by MediaLab Inc. under license from Educational Materials for Health Professionals Inc. Dayton OH, 45420. Copyright EMHP.
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