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Body Fluid Differential Tutorial (Online CE Course)

(based on 3960 customer ratings)

Author: MaryBeth Helfrich, MT(ASCP)
Reviewer: Paul Fekete, MD, FCAP

If you have forgotten the art of differentiating choroid plexus cells from ependymal cells or viral lymphocytes from fluid monocytes, you are in luck! This course provides a thorough morphologic identification of over 40 cell types and disease states to recharge your memory. The course features over 100 crystal clear, perfectly stained body fluid images to illustrate both basic and advanced cellular morphology - perfect for a comprehensive, detailed review which can help refresh any technologist, both in or out of the hematology laboratory. The course is P.A.C.E. approved for 2 full credit hours to help reach your continuing education credit requirements. Enjoy learning and/or reviewing the key identification characteristics of body fluid cells in a user-friendly and fun format. Enroll today!

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Continuing Education Credits

  • P.A.C.E.® Contact Hours (acceptable for AMT, ASCP, and state recertification): 2 hour(s)
  • Florida Board of Clinical Laboratory Science CE - General (Hematology): 2 hour(s)

Objectives

  • Compare and contrast the morphology of cells found in normal cerebrospinal fluid (CSF), normal pleural fluid, normal peritoneal fluid, and normal synovial fluid.
  • Identify the morphology of cells found in bronchial alveolar lavage (BAL).
  • Distinguish abnormal/reactive/infected cell morphology in fluids.
  • Recognize malignant cell morphology in body fluids.
  • Discuss appropriate scenarios for hematology/pathology review.

Customer Ratings

(based on 3960 customer ratings)

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Course Outline

  • Cytospin Morphology
      • Peripheral Morphology vs Cytospin Morphology
  • Cell Types Common To Most Types of Body Fluids
    • Neutrophils
      • Neutrophils
      • Neutrophils continued
      • Eosinophils vs. Neutrophils
      • The cytospin technique perfectly preserves the morphology of blood cells in a fluid sample.
    • Lymphocytes, Atypical Lymphs, Plasma Cells
      • Normal Lymphocytes
      • Lymphocytes
      • Lymphocytes vs. Monocytes
      • Viral Lymphocytes vs. Monocytes
      • Lymphocytes and Atypical Lymphocytes
      • Atypical Lymphocytes
      • Viral Lymphocytes
      • Plasma Cells
      • A patient with an infectious mononucleosis infection presents in the emergency room. Physicians order a spinal tap which is immediately sent to the la...
    • Monocytes, Macrophages and Histocytes
      • Monocytes
      • Monocytes vs Lymphocytes
      • Monocytes and Macrophages
      • Macrophages
      • Hemophagocytosis
      • Siderophage
      • Histiocytes
      • Macrophages are actually lymphocytes that have entered the tissues and body fluids via diapedesis.
      • Select the specific cells listed below that can be found in all types of body fluid.
  • Cells Found in Cerebrospinal Fluid
    • Cerebrospinal Fluid: Non Blood Cells
      • Ependymal Cells
      • Ependymal Clumps
      • Choroid Plexus Cells
    • Cerebrospinal Fluid: Bone Marrow Contamination
      • Bone Marrow Contamination in Cerebrospinal Fluid
      • What is the identification of this cellular clump found in CSF? Note the presence of many similar-appearing nuclei without distinct lines of demarcati...
  • Cells Found in Pleural and Peritoneal Fluids
    • Mesothelial cells
      • Mesothelial Cells
      • Mesothelial Cells continued
      • Reactive Mesothelial Cells
  • Cells Found in Synovial Fluids
      • Synovial Lining Cells
      • Synovial Lining Cells
  • Cells Found in Bronchial Alveolar Lavages
    • Bronchial Lining Cells
      • Bronchial Lining Cells
      • Bronchial Lining Cells continued
      • Match the following blood cell types to the respective body fluids where they may reside.
  • Infectious Organisms in Body Fluids
      • Infectious Organisms in Body Fluids
    • Bacteria
      • Intracellular and Extracellular Diplococci
      • Intracellular Diplococci
      • Peritoneal lavage Trauma STAT
      • Trauma STAT
      • Septic Arthritis
    • Fungus
      • Yeast
      • Candida albicans - Germ Tube Formation
      • Hyphae
    • Toxoplasma
      • Central Nervous System (CNS) Toxoplasmosis
      • Central Nervous System (CNS) Toxoplasmosis continued
      • Identification of tuberculosis can be performed by examination of Wright stained cytospin slides of infected body fluids.
  • Malignant Fluids with Leukemia and Lymphoma.
      • Leukemic Fluids
    • Lymphoid Leukemia / Lymphomas
      • Acute Lymphoblastic Leukemia (ALL): L1 Morphology
      • Central Nervous System (CNS) Relapse: L2 Acute Lymphoblastic Leukemia (ALL)
      • L3 Burkitt Lymphoma
      • Non-Hodgkin Lymphoma
      • Anaplastic Large Cell Lymphoma (ALCL)
    • Myeloid Leukemias
      • Acute Myeloid Leukemia (AML)
      • Acute Myeloid Leukemia (AML) continued
      • Acute Monoblastic Leukemia (AMoL)
      • Prominent vacuolation involving the cytoplasm of abnormal lymphoblast-like cells seen in a body fluid preparation is a distinctive feature of Burkitt ...
  • Malignant Fluids with Metastatic Tumor
      • Metastatic Tumors in Fluid Cytospins.
      • Adenocarcinoma in Peritoneal Fluid
      • Alveolar Rhabdomyosarcoma (ARMS) in Plerual Fluid
      • Atypical Teratoid/Rhabdoid Tumors (ATRT) in Cerebrospinal Fluid
      • Metastatic Melanoma in the Cerebrospinal Fluid
      • Agranular Metastatic Melanoma in Cerebrospinal Fluid
      • Medulloblastoma in Cerebrospinal Fluid
      • Neuroblastoma in Pleural Fluid (NBL)
      • Neuroblastoma Tumor Clump vs. Mesothelial Clump in Pleural Fluid
      • Primitive Neuroectodermal Tumor (PNET) in Cerebrospinal Fluid
      • Retinoblastoma in Cerebrospinal Fluid
      • When evaluating a potentially malignant effusion, select all of the following choices which would apply.
  • References
      • References

Additional Information

Level of Instruction: Intermediate
 
Intended Audience: This course is intended for laboratory professionals who have experience with peripheral blood morphology and basic experience with body fluid differential analysis.  This tutorial will provide a review of normal and abnormal body fluid morphology utilizing Wright-Giemsa stained cytospin preparations from cerebrospinal fluid (CSF), pleural, peritoneal and synovial fluids as well as bronchial alveolar lavage (BAL) samples.
 
Author Information: Marybeth Helfrich, MT(ASCP) is currently a Laboratory Technologist Specialist for the Hematology/Oncology Laboratory at Childrens Hospital of Philadelphia. She received her BS, MT from Temple University in Philadelphia and has nearly 40 years of experience in both adult and pediatric hematology and oncology settings. She is a regular presenter for ASCLS-PA, has been a presenter for ASCP (local and national), and the Texas Teleconference network. She is responsible for hematology morphology instruction and training for medical technologists and fellows. She is also a regular developer of morphology tutorials for in-house continuing education activities.

Reviewer Information: Paul Fekete, MD, FCAP is the President and CEO of MediaLab, Inc. He received his MD from Ohio State University and completed his clinical pathology residency at Emory University in Atlanta. He has served as an Assistant Professor of Pathology at Emory and as the Director of Laboratories for Gwinnett Medical Center in Lawrenceville, GA. He is currently President of Gwinnett Pathology Associates PC, Gwinnett Medical Center. Dr. Fekete is a member of Alpha Omega Alpha Honor Medical Society and a Fellow of the College of American Pathologists.





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csf 10 sml


csf atyp lymph vs mono sml2


corrected normal csf 1 sml2


lymhoblasts sml


synovial clump sml


trauma pert lavage sml2


burketts sml